6-Thioguanine and S6-Methylthioguanine Are Mutagenic in Human Cells
نویسندگان
چکیده
منابع مشابه
Metabolic effects of 6-thioguanine. I. Studies on thioguanine-resistant and-sensitive Ehrlich ascites cells.
The guanine analog, 6-thioguanine (4), has been found to be a potent inhibitor of animal neoplasms (2) and human leukemia (5) when administered alone, and even more potent when used in com bination with the inhibitor of de novo purine syn thesis, azaserine (10, 12). We have developed a thioguanine-resistant subline of the Ehrlich ascites carcinoma. Using this as a tool, we have studied the meta...
متن کاملOxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells.
The thiopurines azathioprine and 6-mercaptopurine have been extensively prescribed as immunosuppressant and anticancer agents for several decades. A third member of the thiopurine family, 6-thioguanine (6-TG), has been used less widely. Although known to be partly dependent on DNA mismatch repair (MMR), the cytotoxicity of 6-TG remains incompletely understood. Here, we describe a novel MMR-inde...
متن کاملDissimilar actions of 6-mercaptopurine and 6-thioguanine in Chinese hamster ovary cells.
The actions of 6-thioguanine (TG) and 6-mercaptopurine (MP) were compared in Chinese hamster ovary (CHO) cells. Several differences were noted between these two agents. TG caused a greater maximal loss of clonogenicity, leaving about one log fewer survivors than did MP, although the cells killed by MP appeared to succumb much more rapidly than those killed by TG. MP-treated populations experien...
متن کاملEffect of 6-Thioguanine and Methyl-6-Thioguanine on stability of DNA duplexes
6-Thioguanine (isG) has been used for the past thirty years as an anti-cancer agent in maintenance chemotherapy for childhood acute lymphoblastic leukemia as well as acute myeloid leukemia. Despite its long-standing clinical use, little is understood of the molecular basis of the cytotoxicity associated with 6SG. Research has shown that 6SG is incorporated into DNA during replication after whic...
متن کاملScheduling of arabinosylcytosine and 6-thioguanine therapy.
SUMMARY Mice treated simultaneously with 1-0-D-arabinofuranosyl-cytosine (ara-C) and 6-thioguanine (6-TG) were protected from the lethal effects of 6-TG. No protection was afforded if ara-C was given 16 hr before the 6-TG. DNA synthesis in mouse bone marrow was immediately depressed to 3 to 12% of that of control in various experiments, after treatment with ara-C, 10 to 20 mg/kg; it then reboun...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2010
ISSN: 1554-8929,1554-8937
DOI: 10.1021/cb100214b